Western blot of forebrain lysates from wildtype and alpha1 knockout animals, using approximately 5-7 ug of tissue per lane. Blots were incubated overnight at 2-8° C with anti-GABAA R, alpha 1 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~51kD alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals (K/O).
Supplier Page from United States Biological for Rabbit Anti-GABA Receptor A, alpha 1 Antibody